8XYF
Crystal structure of Holo-PlyGRCS, a bacteriophage Endolysin in complex with Cold shock protein C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97373 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.100, 58.004, 75.928 |
| Unit cell angles | 90.00, 121.55, 90.00 |
Refinement procedure
| Resolution | 25.160 - 1.670 |
| R-factor | 0.1749 |
| Rwork | 0.173 |
| R-free | 0.20990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.911 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.131 | 1.010 |
| Number of reflections | 35134 | 35134 |
| <I/σ(I)> | 19.6 | |
| Completeness [%] | 99.2 | 93.8 |
| Redundancy | 5.5 | 3.2 |
| CC(1/2) | 0.991 | 0.605 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM MES, pH 6.0, 35% PEGMME 5K |
