8XX1

The Crystal Structure of MAPKAP kinase 2 domain from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953732
Spacegroup name	P 63 2 2
Unit cell lengths	103.239, 103.239, 166.788
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.310 - 2.550
Rwork	0.166
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.622
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.310	2.660
High resolution limit [Å]	2.550	2.550
Number of reflections	17831	2110
<I/σ(I)>	18.2	4.7
Completeness [%]	100.0
Redundancy	21.5
CC(1/2)	0.998	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.1M Sodium malonate dibasic monohydrate, 0.1M HEPES pH 7, 0.5% v/v Jeffamine ED2003