8XWZ

Non-crystalline proximal tubulopathy and crystalline cast nephropathy-causing Bence-Jones protein PT-CN: An entire immunoglobulin kappa light chain dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-06
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.04
Spacegroup name	P 41 21 2
Unit cell lengths	80.422, 80.422, 141.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.290 - 2.300
R-factor	0.2071
Rwork	0.204
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b6d
RMSD bond length	0.002
RMSD bond angle	0.559
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.080	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	21369	2051
<I/σ(I)>	12.5	1.5
Completeness [%]	100.0	100
Redundancy	13.2	11.3
CC(1/2)	0.999	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION	6.5	283	100 mM HEPES, 8% MPD, 10% PEG6000