8XWY

Structure of Interleukin-27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9870
Spacegroup name	P 61 2 2
Unit cell lengths	130.450, 130.450, 260.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.350 - 3.400
R-factor	0.258
Rwork	0.254
R-free	0.29720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.595
Data reduction software	XDS (1.15.2_3472)
Data scaling software	XDS (VERSION Feb 5, 2021)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	3.521
High resolution limit [Å]	3.400	3.400
Number of reflections	33328	1356
<I/σ(I)>	12
Completeness [%]	97.6	99.7
Redundancy	23.6
CC(1/2)	0.999	0.447

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.05M Lithium sulfate, 0.05M Sodium sulfate,0.05M Tris-HCl pH 8.5, 35%PEG 400