8XWX

Crystal structure of FIS1-BAP31 complex from human

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	C 2 2 2
Unit cell lengths	104.632, 237.267, 90.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.960 - 2.690
R-factor	0.256
Rwork	0.254
R-free	0.28680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.782
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.21rc1_5156)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.960	2.760
High resolution limit [Å]	2.690	2.690
Rmeas	0.050
Rpim	0.018	0.587
Number of reflections	27276	946
<I/σ(I)>	99.8
Completeness [%]	85.8	42.16
Redundancy	8.2
CC(1/2)	0.993	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	287	100 mM sodium acetate pH 4.6, 1.5 M lithium sulfate