8XUW

Crystal Structure of the bromodomain of Fusarium graminearum GCN5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2020-11-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	65.582, 74.977, 50.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 1.580
Rwork	0.189
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.252
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (v1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.910	1.610
High resolution limit [Å]	1.580	1.580
Number of reflections	17567	824
<I/σ(I)>	26.9
Completeness [%]	99.8
Redundancy	12.5
CC(1/2)	1.000	0.642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.2 M Sodium chloride,0.1 M BIS-TRIS pH5.5,25% w/v Polyethylene glycol 3350