8XUP
Crystal structure of lipoprotein NlpI in complex with MepS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-09 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 197.193, 99.439, 196.244 |
| Unit cell angles | 90.00, 104.45, 90.00 |
Refinement procedure
| Resolution | 29.700 - 2.800 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.391 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.073 | 0.458 |
| Rmeas | 0.086 | 0.544 |
| Rpim | 0.044 | |
| Number of reflections | 90404 | 8940 |
| <I/σ(I)> | 15.39 | |
| Completeness [%] | 99.9 | 98.8 |
| Redundancy | 3.8 | 3.3 |
| CC(1/2) | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 1.4M sodium acetate, 0.1M sodium cacodylate, pH 6.5 |
