8XUL

Structure of beta-1,2-glucanase from Xanthomonas campestris pv. campestris (beta-1,2-glucoheptasaccharide complex)-E239Q mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 43 3 2
Unit cell lengths	220.178, 220.178, 220.178
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.233 - 2.500
Rwork	0.338
R-free	0.36460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.059
RMSD bond angle	2.011
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.233	2.510
High resolution limit [Å]	2.450	2.450
Rmerge	0.121	1.050
Rpim	0.027	0.233
Number of reflections	67208	4460
<I/σ(I)>	33.7	5
Completeness [%]	100.0
Redundancy	39.1
CC(1/2)	1.000	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	NaNO3, PEG 3350, Bis-tris propane