8XUL
Structure of beta-1,2-glucanase from Xanthomonas campestris pv. campestris (beta-1,2-glucoheptasaccharide complex)-E239Q mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 220.178, 220.178, 220.178 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.233 - 2.500 |
Rwork | 0.338 |
R-free | 0.36460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.059 |
RMSD bond angle | 2.011 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.233 | 2.510 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.121 | 1.050 |
Rpim | 0.027 | 0.233 |
Number of reflections | 67208 | 4460 |
<I/σ(I)> | 33.7 | 5 |
Completeness [%] | 100.0 | |
Redundancy | 39.1 | |
CC(1/2) | 1.000 | 0.949 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | NaNO3, PEG 3350, Bis-tris propane |