8XUJ

Structure of beta-1,2-glucanase from Endozoicomonas elysicola (EeSGL1, ligand-free)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	110.502, 114.908, 78.885
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.442 - 2.400
Rwork	0.224
R-free	0.28080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.420
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.442	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.141	0.932
Rpim	0.057	0.372
Number of reflections	40027	4131
<I/σ(I)>	14.1	2.9
Completeness [%]	100.0	100
Redundancy	13.2	13.9
CC(1/2)	0.998	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	potassium thiocyanate, Bis-tris propane, PEG 3350