8XUF

CDF1 Dof domain in palindromic-bound complex with DNA duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2021-11-06
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	79.442, 79.442, 93.729
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.530 - 2.300
R-factor	0.2301
Rwork	0.229
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.002
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.530	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.053	1.359
Rmeas	0.056	1.413
Rpim	0.015	0.382
Number of reflections	13900	1344
<I/σ(I)>	34.42	2.29
Completeness [%]	99.8	100
Redundancy	12.9	13.4
CC(1/2)	1.000	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES-NaOH, 50 mM magnesium chloride hexahydrate and 30% polyethylene glycol (PEG) 550 monomethyl ether (MME)