8XU7

Crystal structure of Lsd18 in complex with a product

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	47.037, 62.234, 76.294
Unit cell angles	74.79, 81.97, 76.81

Refinement procedure

Resolution	19.612 - 2.200
R-factor	0.2291
Rwork	0.227
R-free	0.27120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8up4
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.612	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.126	0.650
Rmeas	0.136	0.708
Rpim	0.052	0.274
Number of reflections	40337	3490
<I/σ(I)>	10.7	3.2
Completeness [%]	98.0	97.3
Redundancy	6.9	6.8
CC(1/2)	0.995	0.332

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291.15	0.1 M Tris pH7.5, 1.0 M NaCl, 32% (w/v) PEG8000, 4% tert-butanol