8XU6

Structural Insights into the Tumor Suppressor ZMYND11 Reveal Diverse Recognition Mechanisms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	198
Detector technology	PIXEL
Collection date	2022-11-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 63 2 2
Unit cell lengths	55.022, 55.022, 74.411
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.650 - 1.628
Rwork	0.181
R-free	0.22650
Structure solution method	AB INITIO PHASING
RMSD bond length	0.009
RMSD bond angle	1.561
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.760	1.660
High resolution limit [Å]	1.628	1.630
Number of reflections	8809	2301
<I/σ(I)>	73.4
Completeness [%]	99.9
Redundancy	19
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	40 % v/v 2-Methyl-2,4-pentanediol,100 mM MES pH 6.5