8XTZ

Crystal structure of Lsd18 in complex with a substrate

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	46.653, 61.769, 75.693
Unit cell angles	74.62, 81.82, 77.04

Refinement procedure

Resolution	25.331 - 1.850
R-factor	0.163
Rwork	0.161
R-free	0.20260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8up4
RMSD bond length	0.006
RMSD bond angle	0.833
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.200	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.116	0.218
Rmeas	0.165	0.261
Rpim	0.116	0.140
Number of reflections	65616	3782
<I/σ(I)>	11.9	5.1
Completeness [%]	97.2	90.3
Redundancy	1
CC(1/2)	0.982	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291.15	0.1 M Tris pH7.5, 1.0 M NaCl, 32% (w/v) PEG8000, 4% tert-butanol