8XT7

The crystal structure of human AS3MT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	85.829, 85.772, 97.852
Unit cell angles	90.00, 104.44, 90.00

Refinement procedure

Resolution	46.860 - 2.590
R-factor	0.2025
Rwork	0.201
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.137
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.380	2.683
High resolution limit [Å]	2.590	2.590
Number of reflections	40586	3849
<I/σ(I)>	9.185	1.611
Completeness [%]	94.4
Redundancy	3
CC(1/2)	0.978	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296.15	PEG 3350, Sodium Malonate pH7.0