8XSQ

Pseudomonas aeruginosa Histidinol dehydrogenase native structure without Zn+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	110
Detector technology	PIXEL
Collection date	2018-06-22
Detector	Bruker PHOTON III
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	66.307, 89.435, 141.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.190 - 1.750
R-factor	0.2062
Rwork	0.206
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	4.631
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.190	1.830
High resolution limit [Å]	1.750	1.750
Rpim		0.468
Number of reflections	82507	8161
<I/σ(I)>	28.1
Completeness [%]	99.0
Redundancy	14.2
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	295	15% PEG 8000, 100 mM Na-Acetate pH 4.6,