8XSG

Crystal structure of the Actinobacillus minor NM305 glucosyltransferase in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	100.136, 43.390, 95.823
Unit cell angles	90.00, 117.44, 90.00

Refinement procedure

Resolution	42.557 - 1.800
Rwork	0.158
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8xsh
RMSD bond length	0.008
RMSD bond angle	1.448
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.034
Number of reflections	32975	1948
<I/σ(I)>	33
Completeness [%]	96.7
Redundancy	7
CC(1/2)	1.000	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES pH 6.5, 0.2 M sodium formate, 14% (w/v) PEG 3350