8XRX

The crystal structure of a GH3 enzyme CcBgl3B with glucose and gentiobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97538
Spacegroup name	C 1 2 1
Unit cell lengths	163.095, 183.009, 269.154
Unit cell angles	90.00, 100.59, 90.00

Refinement procedure

Resolution	19.948 - 2.500
R-factor	0.1967
Rwork	0.197
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.960
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.102	0.538
Number of reflections	264973	11550
<I/σ(I)>	8.2
Completeness [%]	99.0
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG