8XRI
The crystal structure of AsfvTopII in complex with both G-DNA and T-DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-10-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 129.210, 134.366, 135.926 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.610 - 2.920 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.24970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.792 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 135.930 | 3.080 |
High resolution limit [Å] | 2.920 | 2.920 |
Rmerge | 0.216 | 2.089 |
Rmeas | 0.233 | 2.288 |
Rpim | 0.085 | 0.918 |
Total number of observations | 393991 | 45965 |
Number of reflections | 52171 | 7538 |
<I/σ(I)> | 8.4 | 1.8 |
Completeness [%] | 100.0 | |
Redundancy | 7.6 | 6.1 |
CC(1/2) | 0.942 | 0.367 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 0.1 M Tris pH8.0, 55% MPD |