8XRI

The crystal structure of AsfvTopII in complex with both G-DNA and T-DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	129.210, 134.366, 135.926
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.610 - 2.920
R-factor	0.207
Rwork	0.205
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.792
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	135.930	3.080
High resolution limit [Å]	2.920	2.920
Rmerge	0.216	2.089
Rmeas	0.233	2.288
Rpim	0.085	0.918
Total number of observations	393991	45965
Number of reflections	52171	7538
<I/σ(I)>	8.4	1.8
Completeness [%]	100.0
Redundancy	7.6	6.1
CC(1/2)	0.942	0.367

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298.15	0.1 M Tris pH8.0, 55% MPD