8XRH
The crystal structure of AsfvTopII ATPase domain in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 85.650, 85.650, 211.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.380 - 1.250 |
| R-factor | 0.1862 |
| Rwork | 0.186 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.056 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.380 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.117 | 1.376 |
| Rmeas | 0.119 | 1.435 |
| Rpim | 0.020 | 0.394 |
| Total number of observations | 4200597 | 116357 |
| Number of reflections | 127111 | 9266 |
| <I/σ(I)> | 19.5 | 1.8 |
| Completeness [%] | 100.0 | |
| Redundancy | 33 | 12.6 |
| CC(1/2) | 0.999 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 15% (v/v) PEG 550MME, 0.1M MES/Sodium hydroxide pH 6.5 |
