8XRG
The crystal structure of AsfvTopII ATPase domain in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979191 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 85.256, 85.256, 209.538 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.720 - 1.610 |
| R-factor | 0.1866 |
| Rwork | 0.186 |
| R-free | 0.20620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.350 | 1.690 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.087 | 1.290 |
| Rmeas | 0.092 | 1.361 |
| Rpim | 0.027 | 0.430 |
| Total number of observations | 661408 | 80434 |
| Number of reflections | 59466 | 8497 |
| <I/σ(I)> | 17.4 | 2.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 11.1 | 9.5 |
| CC(1/2) | 0.999 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M Potassium phosphate dibasic, 20% w/v Polyethylene glycol 3,350 |
