8XRG
The crystal structure of AsfvTopII ATPase domain in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979191 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 85.256, 85.256, 209.538 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.720 - 1.610 |
R-factor | 0.1866 |
Rwork | 0.186 |
R-free | 0.20620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.082 |
Data reduction software | autoPROC |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.350 | 1.690 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.087 | 1.290 |
Rmeas | 0.092 | 1.361 |
Rpim | 0.027 | 0.430 |
Total number of observations | 661408 | 80434 |
Number of reflections | 59466 | 8497 |
<I/σ(I)> | 17.4 | 2.6 |
Completeness [%] | 99.9 | |
Redundancy | 11.1 | 9.5 |
CC(1/2) | 0.999 | 0.630 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M Potassium phosphate dibasic, 20% w/v Polyethylene glycol 3,350 |