8XQK

The Crystal Structure of Apaf from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1806
Spacegroup name	P 2 21 21
Unit cell lengths	31.641, 75.336, 161.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.696 - 2.850
Rwork	0.225
R-free	0.28310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.965
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.750	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.036	0.843
Number of reflections	18178	1113
<I/σ(I)>	29.9
Completeness [%]	100.0
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6M Na/KPO4 pH6.5