8XPW

Marine bacterial laminarinase PtLam treated with 100 mM EDTA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-10
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54184
Spacegroup name	P 21 21 21
Unit cell lengths	77.449, 77.598, 89.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.530 - 2.650
R-factor	0.21989
Rwork	0.218
R-free	0.25259
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.530	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.408	1.239
Rmeas	0.430	1.314
Rpim	0.135	0.432
Total number of observations	159989	19216
Number of reflections	16196	2122
<I/σ(I)>	5.9	2.1
Completeness [%]	99.8
Redundancy	9.9	9.1
CC(1/2)	0.976	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	400 mM potassium phosphate dibasic, 1600 mM sodium phosphate monobasic and 100 mM sodium phosphate dibasic/citric acid pH 4.2