8XPW
Marine bacterial laminarinase PtLam treated with 100 mM EDTA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-10 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.449, 77.598, 89.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.530 - 2.650 |
| R-factor | 0.21989 |
| Rwork | 0.218 |
| R-free | 0.25259 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.530 | 2.780 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.408 | 1.239 |
| Rmeas | 0.430 | 1.314 |
| Rpim | 0.135 | 0.432 |
| Total number of observations | 159989 | 19216 |
| Number of reflections | 16196 | 2122 |
| <I/σ(I)> | 5.9 | 2.1 |
| Completeness [%] | 99.8 | |
| Redundancy | 9.9 | 9.1 |
| CC(1/2) | 0.976 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 400 mM potassium phosphate dibasic, 1600 mM sodium phosphate monobasic and 100 mM sodium phosphate dibasic/citric acid pH 4.2 |
