8XPK
Marine bacterial laminarinase PtLam mutant E154A in complex with laminatriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-04 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 77.313, 77.313, 89.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.510 - 2.000 |
| R-factor | 0.19012 |
| Rwork | 0.188 |
| R-free | 0.22765 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.510 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.312 |
| Rmeas | 0.093 | 0.353 |
| Rpim | 0.031 | 0.162 |
| Total number of observations | 287223 | 12446 |
| Number of reflections | 36940 | 2721 |
| <I/σ(I)> | 14.7 | 4.2 |
| Completeness [%] | 99.8 | |
| Redundancy | 7.8 | 4.6 |
| CC(1/2) | 0.997 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 3350 and 0.2 M K thiocyanate |
