8XPI

Structure of the C-terminal domain of nsp4 from PEDV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 61
Unit cell lengths	58.815, 58.815, 52.014
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.410 - 1.840
R-factor	0.2033
Rwork	0.201
R-free	0.24510
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.341
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.940	1.940
High resolution limit [Å]	1.840	1.840
Rmerge	0.065	1.220
Number of reflections	9006	1281
<I/σ(I)>	21
Completeness [%]	99.9
Redundancy	17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	800 mM Succinic acid pH 7.0