8XPH

Marine Planctomycetes laminarinase PtLam

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-17
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54184
Spacegroup name	P 2 21 21
Unit cell lengths	77.096, 77.336, 90.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.470 - 2.200
R-factor	0.22323
Rwork	0.221
R-free	0.26652
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.470	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.145	0.303
Rmeas	0.163	0.381
Rpim	0.071	0.225
Total number of observations		6313
Number of reflections	27872	2473
<I/σ(I)>	6.7	2.3
Completeness [%]	99.5
Redundancy	4.6	2.6
CC(1/2)	0.980	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% (w/v) PEG 3000, 100 mM Tris base/ Hydrochloric acid pH 7.0, 200 mM Calcium acetate