8XOV
The Crystal Structure of N-terminal kinase domain of human RSK-1 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 33.911, 54.346, 207.028 |
| Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
| Resolution | 33.700 - 2.550 |
| Rwork | 0.203 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.572 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.700 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.069 | 0.456 |
| Number of reflections | 11642 | 1470 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 93.8 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MgAC2, 0.1M Nacacodylate pH6.5, 15% PEG 6000 |
