Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.935, 116.695, 201.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.940 - 2.750 |
| Rwork | 0.203 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.489 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.1.17.3360) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.940 | 2.830 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.146 | 0.619 |
| Number of reflections | 59507 | 4562 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 24% polyethlene glycol 400, 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate, pH 5.5 |
