8XOC
beta-1,4-galacosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.148, 57.260, 77.180 |
| Unit cell angles | 90.00, 116.49, 90.00 |
Refinement procedure
| Resolution | 45.880 - 1.870 |
| R-factor | 0.21024 |
| Rwork | 0.208 |
| R-free | 0.24687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.427 |
| Data reduction software | XDS (February 5, 2021) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.880 | 1.910 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 44062 | 2837 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.995 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Magnesium acetate tetrahydrate, 0.1M Sodium cacodylate trihydrate, 20% PEG 8000 |
