8XO8
Crystal structure of measles virus fusion inhibitor MEK35GT complexed with F protein HR1 (HR1-42) (P21 space group)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999990 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.747, 53.577, 109.051 |
| Unit cell angles | 90.00, 94.96, 90.00 |
Refinement procedure
| Resolution | 48.005 - 1.846 |
| Rwork | 0.180 |
| R-free | 0.23960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.402 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.846 | 1.846 |
| Rmeas | 0.051 | 0.482 |
| Number of reflections | 36255 | 5761 |
| <I/σ(I)> | 17.13 | 2.78 |
| Completeness [%] | 99.3 | 98.9 |
| Redundancy | 3.45 | 3.53 |
| CC(1/2) | 0.999 | 0.865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% (v/v) 2-propanol, 20% (w/v) PEG 4000, 100 mM HEPES (pH 7.5) |
