8XNE

Crystal structure of measles virus fusion inhibitor M1 complexed with F protein HR1 (HR1-42) (H32 space group)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000000
Spacegroup name	H 3 2
Unit cell lengths	28.506, 28.506, 387.637
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.601 - 1.155
Rwork	0.160
R-free	0.18710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.813
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.220
High resolution limit [Å]	1.155	1.155
Rmeas	0.096	0.446
Number of reflections	22473	3484
<I/σ(I)>	15.71	5.12
Completeness [%]	99.6	98.3
Redundancy	8.72	7.94
CC(1/2)	0.998	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	200 mM MgCl2, 30% (w/v) PEG 3350, 100 mM MES (pH 6.5)