8XM1
Phytase mutant APPAmut4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.514, 46.016, 192.797 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.350 - 1.900 |
R-factor | 0.1607 |
Rwork | 0.158 |
R-free | 0.20810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.918 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.350 | 1.960 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 31168 | 2732 |
<I/σ(I)> | 8.95 | 2.46 |
Completeness [%] | 98.2 | |
Redundancy | 2 | |
CC(1/2) | 0.993 | 0.876 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1 M Sodium acetate trihydrate pH 4.5; 30% w/v Polyethylene glycol 1,500 |