8XKD
beta-1,4-galacosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97851 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.600, 92.810, 71.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.710 - 1.730 |
| R-factor | 0.20212 |
| Rwork | 0.200 |
| R-free | 0.23359 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.446 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.710 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 57948 | 3113 |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.9 | |
| CC(1/2) | 0.993 | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.26M Sodium phosphate monobasic monohydrate, 0.14M Potassium phosphate dibasic |
