8XK8
N1D10 Fab bound to SFTSV glycoprotein-Gn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 84.124, 111.909, 87.856 |
| Unit cell angles | 90.00, 94.33, 90.00 |
Refinement procedure
| Resolution | 20.690 - 3.530 |
| R-factor | 0.2539 |
| Rwork | 0.250 |
| R-free | 0.32390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.799 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.160 | 3.850 |
| High resolution limit [Å] | 3.520 | 3.520 |
| Number of reflections | 20028 | 4658 |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 98.5 | |
| Redundancy | 2.7 | 2.6 |
| CC(1/2) | 0.758 | 0.626 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.095 M tri-Sodium citrate pH 5.6, 19 %(v/v) Isopropanol, 19 %(w/v) PEG 4000, 5 %(v/v) glycerol |
