8XK6

S2A5 Fab bound to SFTSV glycoprotein Gn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-27
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	P 63 2 2
Unit cell lengths	158.020, 158.020, 190.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.490 - 2.450
R-factor	0.2317
Rwork	0.231
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.530
High resolution limit [Å]	2.450	2.450
Number of reflections	50670	4647
<I/σ(I)>	15.6
Completeness [%]	98.0
Redundancy	13.6
CC(1/2)	0.999	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	40 % (v/v) pentaerythritol propoxylate (5/4 PO/OH), 0.2 M sodium thiocyanate, 0.1 M HEPES pH7.0