8XJH

Crystal structure of Arabidopsis N-amino acetyltransferase 2 (AtNATA2) bound to di-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	55.450, 85.550, 86.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.670 - 1.750
R-factor	0.18955
Rwork	0.188
R-free	0.22604
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.670	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	41905	4113
<I/σ(I)>	13.46
Completeness [%]	99.9
Redundancy	14.3
CC(1/2)	0.990	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	0.1 M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2,000