8XJG

Crystal structure of the YqeY protein from Vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-07-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	43.721, 107.577, 34.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.140 - 1.700
R-factor	0.2281
Rwork	0.227
R-free	0.25780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.945
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	18735	912
<I/σ(I)>	40	2.3
Completeness [%]	99.8	99.7
Redundancy	6.4	5.8
CC(1/2)	0.996	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	PEG 8000, zinc acetate, MES