8XJC

a novel haemophore of of Riemerella anatipestifer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 2
Unit cell lengths	110.480, 120.060, 53.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.030 - 2.850
R-factor	0.2281
Rwork	0.225
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.668
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.030	2.920
High resolution limit [Å]	2.850	2.850
Rmerge	0.093
Number of reflections	17335	16827
<I/σ(I)>	7.63
Completeness [%]	97.0
Redundancy	11.2
CC(1/2)	0.971	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	PEG3350, Ammonium citrate