8XHD

Crystal structure of alpha-Oxoamine Synthase Alb29 with PLP cofactor and L-glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 32 2 1
Unit cell lengths	117.734, 117.734, 145.018
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.680 - 2.700
R-factor	0.2172
Rwork	0.215
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8xha
RMSD bond length	0.002
RMSD bond angle	0.541
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.960	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.108	1.274
Rmeas	0.113	1.306
Rpim	0.033	0.389
Total number of observations	368062
Number of reflections	32498	3210
<I/σ(I)>	20.3
Completeness [%]	100.0	99.97
Redundancy	11.3	11.5
CC(1/2)	0.999	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		293.15	0.1 M Amino acid mix, 0.1 M Buffer system 3, pH8.5, 30% v/v Precipitant Mix2