8XHA

Crystal structure of alpha-Oxoamine Synthase Alb29 with PLP cofactor and L-glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 32 2 1
Unit cell lengths	117.369, 117.369, 145.106
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.990 - 2.270
R-factor	0.1911
Rwork	0.189
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.905
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.351
High resolution limit [Å]	2.270	2.270
Rmerge	0.078	1.260
Rmeas	0.080
Rpim	0.018
Number of reflections	53844	5293
<I/σ(I)>	26.95	2.44
Completeness [%]	99.9	100
Redundancy	8.7
CC(1/2)	0.992	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	8.5	293.15	0.1 M Amino acid mix, 0.1 M Buffer system 3, pH8.5, 30% v/v Precipitant Mix2;