8XH0

Monoclinic crystal structure of green fluorescent protein nowGFP at pH 4.8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-10-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	C 1 2 1
Unit cell lengths	110.547, 51.039, 55.515
Unit cell angles	90.00, 99.58, 90.00

Refinement procedure

Resolution	29.620 - 1.450
R-factor	0.12869
Rwork	0.127
R-free	0.15823
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	2.101
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.8.0267)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.480
High resolution limit [Å]	1.450	1.450
Rmerge	0.072	0.688
Rmeas	0.077	0.742
Rpim	0.029	0.275
Number of reflections	54859	2715
<I/σ(I)>	37.3	2.5
Completeness [%]	99.9	100
Redundancy	7.3	7.2
CC(1/2)	1.000	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277	16mM KH2PO4 pH 4.8, 20% (w/v) PEG 3350