8XGX

beta-1,4-galacosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-29
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97628
Spacegroup name	P 42 21 2
Unit cell lengths	87.840, 87.840, 140.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.540 - 2.410
R-factor	0.21057
Rwork	0.208
R-free	0.25261
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.329
Data reduction software	XDS (February 5, 2021)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.750	2.500
High resolution limit [Å]	2.410	2.410
Number of reflections	21910
<I/σ(I)>	13.8
Completeness [%]	100.0
Redundancy	25.7
CC(1/2)	0.999	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.26M Sodium phosphate monobasic monohydrate, 0.14M Potassium phosphate monobasic