8XGQ
The co-crystal structure of LtpM with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2020-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.593, 64.925, 84.423 |
| Unit cell angles | 109.92, 94.24, 109.06 |
Refinement procedure
| Resolution | 28.290 - 3.040 |
| R-factor | 0.2618 |
| Rwork | 0.257 |
| R-free | 0.30140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Alphafold |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.087 | 0.466 |
| Number of reflections | 19903 | 1830 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 96.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.995 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.44 M ammonium sulfate, 0.1 M BIS-TRIS pH 6.4, 0.1 M NaCl |
