8XGL
Structure of the Magnaporthe oryzae effector NIS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 |
| Unit cell lengths | 129.800, 129.800, 79.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.120 - 2.300 |
| R-factor | 0.1782 |
| Rwork | 0.177 |
| R-free | 0.19430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.702 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.120 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 22307 | 2237 |
| <I/σ(I)> | 11.42 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8.3 | |
| CC(1/2) | 0.992 | 0.594 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M BIS-Tris (pH 6.5), 2.0 M ammonium sulfate |
