8XG6
X-ray crystal structure of Streptavidine without cryo-protectant using a high-pressure cryocooling method
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.941, 85.948, 58.389 |
| Unit cell angles | 90.00, 99.21, 90.00 |
Refinement procedure
| Resolution | 31.509 - 1.310 |
| R-factor | 0.1471 |
| Rwork | 0.145 |
| R-free | 0.18240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.780 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.870 | 1.330 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Number of reflections | 109516 | 5389 |
| <I/σ(I)> | 13.1 | 1.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 4.2 | |
| CC(1/2) | 0.999 | 0.656 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.0, 20% PEG 1000 |
