8XG5
X-ray crystal structure of streptavidin flash-cooled in 30% PEG1000 at ambient pressure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.752, 84.946, 58.345 |
| Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
| Resolution | 33.574 - 1.400 |
| R-factor | 0.148 |
| Rwork | 0.146 |
| R-free | 0.18260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.791 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.690 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 88033 | 4382 |
| <I/σ(I)> | 13.3 | 1.5 |
| Completeness [%] | 99.5 | |
| Redundancy | 4.2 | 4.1 |
| CC(1/2) | 0.999 | 0.712 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.0, 20% PEG 1000 |
