Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979514 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.063, 273.373, 170.341 |
| Unit cell angles | 90.00, 92.14, 90.00 |
Refinement procedure
| Resolution | 48.213 - 3.200 |
| Rwork | 0.229 |
| R-free | 0.30270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.737 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.213 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.230 | 0.947 |
| Number of reflections | 50864 | 4397 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 99.4 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M sodium malonate pH6.75, 18% PEG 3350 |
