8XFW

Crystal structure of MiCGT(M148A/V190T/S121D) in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-07
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	93.194, 103.617, 110.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.970 - 3.100
R-factor	0.195197003472
Rwork	0.192
R-free	0.23948
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.818
Data reduction software	HKL-3000
Phasing software	PHASES
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.970	3.211
High resolution limit [Å]	3.100	3.100
Number of reflections	37033	6919
<I/σ(I)>	9.17
Completeness [%]	99.8
Redundancy	10.9
CC(1/2)	0.980	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION	7	277	200mM calcium acetate,100mM tris (PH 6.5-7.5),PEG 3000 16%-22%