8XFH

Crystal structure of MiCGT(E152Q/V190D/S122P) in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-07
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	92.532, 103.354, 108.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.494 - 2.800
R-factor	0.222902956102
Rwork	0.221
R-free	0.24411
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.834
Data reduction software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.150	3.005
High resolution limit [Å]	2.800	2.902
Number of reflections	26288	6917
<I/σ(I)>	2.1
Completeness [%]	99.3
Redundancy	12.8
CC(1/2)	0.969	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	200mM calcium acetate,100mM tris (PH 6.5-7.5),PEG 3000 16%-22%