8XF9

High-resolution structure of the siderophore periplasmic binding protein FtsB mutant Y137A from Streptococcus pyogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-23
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0000
Spacegroup name	P 65
Unit cell lengths	75.847, 75.847, 102.622
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.400 - 1.150
Rwork	0.131
R-free	0.14680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.623
Data reduction software	XDS (Feb 5, 2021 BUILT=20210323)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.400	1.170
High resolution limit [Å]	1.150	1.150
Rmerge	0.062	1.190
Rpim	0.014	0.341
Number of reflections	118354	5830
<I/σ(I)>	24.6	2.3
Completeness [%]	100.0
Redundancy	19.4
CC(1/2)	1.000	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	50 mM Zinc sulfate 100 mM MES pH 6.5 25% PEG MME-550