8XF7

High-resolution structure of the siderophore periplasmic binding protein FtsB from Streptococcus pyogenes with ferrioxamine E bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-27
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0000
Spacegroup name	P 65
Unit cell lengths	76.091, 76.091, 102.375
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.420 - 1.120
Rwork	0.125
R-free	0.14030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8xeu
RMSD bond length	0.008
RMSD bond angle	1.637
Data reduction software	XDS (Jan 10, 2022 BUILT=20220120)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.420	1.140
High resolution limit [Å]	1.120	1.120
Rmerge	0.047	0.961
Rpim	0.011	0.326
Number of reflections	128565	6276
<I/σ(I)>	18.6	2.3
Completeness [%]	99.9	98.9
Redundancy	18.6	9.3
CC(1/2)	0.750	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M HEPES pH 7.5 25% w/v Polyethylene glycol 3,350